Proteins don’t usually work in isolation, but rather make up larger complexes such as molecular machines that enable cells to communicate with each other, move cargo around in their interiors, or replicate their DNA.Yet even with the advent of super-resolution microscopy, the technology has not been powerful enough to distinguish individual molecular features within those densely packed complexes. Up to now, researchers only have been able to visualize closely positioned molecules or molecular complexes with 10 to 20 nanometer resolution.But by advancing technology, a team at Harvard’s Wyss Institute for Biologically Inspired Engineering has been able to distinguish features 5 nanometers from each other in a densely packed, single-molecular structure, achieving the highest resolution in optical microscopy. The team, led by Wyss core faculty member Peng Yin, used “discrete molecular imaging” (DMI), which enhances its DNA nanotechnology-powered, super-resolution microscopy platform with an integrated set of new imaging methods. The study was reported in Nature Nanotechnology on July 4.Super Resolution Discrete Molecular Imaging Animation <a href=”https://www.youtube.com/watch?v=5ChCYOEQwTc” rel=”nofollow” target=”_blank”> <img src=”https://img.youtube.com/vi/5ChCYOEQwTc/0.jpg” alt=”0″ title=”How To Choose The Correct Channel Type For Your Video Content ” /> </a> <a href=”https://www.youtube.com/watch?v=iLyTEb4qYz8″ rel=”nofollow” target=”_blank”> <img src=”https://img.youtube.com/vi/iLyTEb4qYz8/0.jpg” alt=”0″ title=”How To Choose The Correct Channel Type For Your Video Content ” /> </a> See in this animation how Discrete Molecular Imaging (DMI) uses DNA nanotechnology to reveal densely packed molecular features in structures as similar in size as single protein molecules. Credit: Wyss Institute at Harvard University.“The ultra-high resolution of DMI advances the DNA-PAINT platform one step further towards the vision of providing the ultimate view of biology,” said Peng Yin, who is also professor of systems biology at Harvard Medical School.Thee DNA-PAINT technologies developed by Yin and his team are based on the transient binding of two complementary short DNA strands: one attached to the molecular target that the researchers aim to visualize, and the other attached to a fluorescent dye. Repeated cycles of binding and unbinding create a very defined blinking behavior of the dye at the target site, which is highly programmable by the choice of DNA strands and has now been further harnessed by the team’s current work to achieve ultra-high resolution imaging.Discrete Molecular Imaging Wyss Institute Core Faculty member Peng Yin and his co-worker Mingjie Dai explain in this video, how Discrete Molecular Imaging (DMI) can be used to enhance their DNA-PAINT super-resolution imaging platform to visualize features on a single-molecule scale. Credit: Wyss Institute at Harvard University“Peng Yin and his team have yet again broken through barriers never before possible by leveraging the power of programmable DNA, not for information storage, but to create nanoscale ‘molecular instruments’ that carry out defined tasks and read out what they analyze. This new advancement to their DNA-powered super-resolution imaging platform is an amazing feat that has the potential to uncover the inner workings of cells at the single-molecule level using conventional microscopes that are available in common biology laboratories,” said Donald Ingber, Judah Folkman Professor of Vascular Biology at Harvard Medical School and the Vascular Biology Program at Boston Children’s Hospital, and professor of bioengineering at the Harvard John A. Paulson School of Engineering and Applied Sciences.The Wyss Institute’s scientists have benchmarked the ultra-high resolution of DMI using synthetic DNA nanostructures. Next, the researchers plan to apply the technology to actual biological complexes such as the protein complex that duplicates DNA in dividing cells or cell surface receptors binding their ligands.To read the full release, visit the Wyss Institute’s website.— Benjamin Boettner, Wyss Institute Communications
Facebook7Tweet0Pin0Submitted by Employment Security DepartmentWashington veterans will speak about their military experiences in honor of Veterans Day at a ceremony from 12:00 p.m. to 1:00 p.m., November 7, on the state capitol campus in Olympia.The public is invited to gather first at the WWII Memorial near the corner of Capitol Way South and 11th Avenue Southeast. Event attendees will then walk to the Winged Victory Monument, the Vietnam Veterans Memorial Wall and conclude at the Korean War Memorial, hearing a speaker and music at each stop.The speakers:Army veteran and Meritorious Service Medal recipient Jennifer Prewitt. She currently works at WorkSource Joint Base Lewis-McChord, helping soldiers transition to civilian life.Steve Severson, Marine Corps veteran. Severson completed two tours of duty in Afghanistan and now connects businesses in Thurston and Lewis counties with veteran employees.Katrina Stensgaard, Army veteran. Stensgaard was an intelligence analyst, serving for over 15 years in Afghanistan, Iraq, Korea and the Philippines. She currently works for the Washington Service Corps.Army veteran Don Meseck. Meseck retired from the Army Reserve after 26 years and 16 overseas assignments. His decorations include the Combat Action Badge and Bronze Star. He currently works as a labor economist with the Employment Security Department.The Veterans Day ceremony is the 28th annual event sponsored by the International Association of Workforce Professionals (IAWP) and the Employment Security Department’s Veteran Employee Resource Group.Billie Wright, Employment Security’s chief human resources officer and a military spouse, will emcee the event. The Washington National Guard will present the colors. Singers Pierre Bell and Jeanette Nelson of Employment Security, and musician Vincent Anderson of the Health Care Authority, will perform patriotic songs. The Spanaway Lake High School Music Company also will perform. Anderson will formally end the ceremony with “Taps.”“IAWP has been a proud sponsor of this ceremony for all of its 28 years,” said Rebekah Wilkes, president of IAWP’s Southwest Sub-chapter. “We’re honored to host this free event, bringing you four Washington veterans to share their thoughts and wisdom this Veterans Day. Please join us!”Flags will be available for participants to place at the memorials.